艾灸对新生小鼠抑郁模型体质量和行为学的影响研究

［摘要］目的　观察艾灸对新生小鼠脑卒中后抑郁模型体质量和行为学结果的影响，了解艾灸治疗新生小鼠脑卒中后抑郁的效果。方法　ICR小鼠，随机分为3组：假手术组、脑卒中后抑郁模型组和艾灸组。采用复合刺激制备脑卒中后抑郁小鼠模型，即于出生后7d给予新生小鼠脑缺血缺氧刺激、并于术后3w始给予21d慢性不可预见性温和应激。采用Open-Field测试小鼠的水平活动及垂直活动得分，蔗糖水摄入量测试小鼠糖水消耗百分比，以及Morris水迷宫测试小鼠的学习记忆力。结果　模型组小鼠体质量减轻，水平活动和直立活动得分均减少、糖水消耗百分比降低以及逃避潜伏期延长；而与模型组相比，艾灸组小鼠体质量增加，水平活动和直立活动得分增多、糖水消耗百分比也升高，而逃避潜伏期缩短。结论　艾灸对新生小鼠脑卒中后抑郁症状具有改善作用。

［关键词］新生小鼠；脑卒中后抑郁；艾灸；蔗糖水摄入量；Morris水迷宫；学习记忆

Effects of moxibustion on body weight and behavioral performance in neonatal post-stroke depression mice

［Abstract］Objective To observe the effect of moxibustion on body weight and behavioral results in post-stroke depression mice.Methods ICR pups were randomly divided into 3 groups，sham group，post-stroke depression(PSD)model group，moxibustion group.The PSD model was induced by combined stimuli，i.e.brain ischemia and hypoxia at 7d postnatal，then 21d of chronic unexpected mild stimulus(CUMS)since 28d postnatal.Open-Field was used to test crossing activity and rearing activity，sucrose consumption test was used to calculate the percentage of sucrose intake，Morris water maze was employed to evaluate learning and memory abilities.Results Compared to mice in the sham group，mice in the PSD group showed that body weight was lowered，crossing scores and rearing scores were decreased，the percentage of su-crose intake was dropped and the escape period was lengthened.Compared to mice in the model group，mice in the moxibustion group showed that body weight was gained，crossing scores and rearing scores were increased，the percentage of sucrose intake was risen and the escape period was shortened.Conclusion Moxibustion improves the symptoms of post-stroke depression.(www.xing528.com)

［Key words］neonatal mice；post-stroke depression；moxibustion；sucrose intake；Morris water maze；learning and memory

脑卒中后抑郁症(post-stroke depression，PSD)是脑血管病的常见并发症，是指脑卒中后出现以快感缺失为核心症状的不同程度的抑郁状态，属于继发性抑郁症的一种［1］。据报道，PSD总发生率在40%～50%［2］。PSD不仅对患者神经功能的恢复产生负面影响，而且加重患者认知损害。然而目前大多研究关注成年后发生的PSD，而对于新生儿缺氧缺血性脑病(hypoxic-ischemic encephalopathy，HIE)后继发的PSD研究报道较少。实际上，新生儿HIE后抑郁症(即PSD)，除产生上述危害外，还可能影响儿童的生长发育，导致生活质量明显下降，给个人和家庭带来无尽的烦恼，也给国家和社会带来不容忽视的危害。据报道，祖国医学的艾灸疗法治疗PSD具有一定的效果［3］。那么，新生小鼠PSD，其体质量和行为学有哪些变化?艾灸对新生小鼠PSD是否具有改善作用?为此，本研究在前期HIE研究［4-6］的基础上，制备PSD小鼠模型，观察艾灸“百会”穴对PSD小鼠体质量、行为学测试结果的影响，为下一步的研究奠定基础。