热敏灸减少大鼠脑皮质CD11b表达，减轻脑缺血再灌注损伤

［摘要］目的　制备局灶性脑缺血性再灌注损伤大鼠模型，观察热敏灸对模型大鼠大脑皮质CD11b表达的影响。方法　雄性SD大鼠51只，采用随机数字表法将大鼠分为假手术组(n=10)、模型组(n=14)、艾灸组(n=27)。艾灸组根据艾灸前后尾温变化再分为非热敏灸组(n=13)和热敏灸组(n=14)。应用线栓法制备局灶性脑缺血再灌注损伤大鼠模型。采用神经功能缺失评分法观察大鼠的行为学表现；光照测痛仪测定大鼠的甩尾潜伏期；TTC染色法观察大鼠的脑梗死面积；免疫组织化学法检测大脑皮质CD11b的表达。结果　术后3 d时，非热敏灸组(9.32±1.11)s与热敏灸组(8.69±0.51)s大鼠甩尾潜伏期均明显短于模型组(12.21±1.04)s，以热敏灸组缩短更为明显；非热敏灸组(20.59±2.25)%和热敏灸组(13.18±3.50)%大鼠脑梗死面积小于模型组(32.22±14.20)%，以热敏灸组缩小更为明显；非热敏灸组(5.40±0.58)和热敏灸组(2.89±0.71)大鼠大脑皮质CD11b表达低于模型组(7.83±1.60)，以热敏灸组降低更为明显。结论　热敏灸具有改善大鼠的感觉功能、减轻大鼠脑缺血再灌注损伤的作用；减少大脑皮质CD11b的表达、减轻炎症反应可能是其作用机制之一。

［关键词］艾炷灸；脑缺血再灌注损伤；CD11b；大鼠；甩尾潜伏期

Heat-sensitive moxibustion decreased the expression of CD11b in cortex of focal cerebral ischemia-reperfusion injury model rats

［Abstract］Objective To observe the effect of heat-sensitive moxibustion on the expression of CD11b in focal cerebral ischemia reperfusion injury model rats.Methods 51 Male SD rats were randomly divided into three groups，a sham-operated group(n=10)，an ischemia-reperfusion(I/R)injury group(n=14)and a moxibustion group(n=27).According to the changes of tail temperature before and after moxibustion，moxibustion group was subdivi-ded into non heat-sensitive moxibustion group(n=13)and heat-sensitive moxibustion group(n=14).Focal cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion for 2 hours and reperfusion for 3 days.Behavioral performance were tested using neurologic deficit scores，Tail-flick latency was determined with Tail Flick Analgesia Meter，infarct areas were examined using TTC staining，and cortical expression of CD11b were measured using immunohistochemical staining.Results After 3 d of operation，the tail-flick latency was significantly shorter in non heat-sensitive moxibustion group(9.32±1.11)s and heat-sensitive moxibustion group(8.69±0.51)s than that of model group(12.21±1.04)s.The area of cerebral infarction was smaller in non heat-sensitive moxibustion group(20.59±2.25)%and heat-sensitive moxibustion group(13.18±3.50)%than that in model group(32.22 ±14.20)%.Compared with model group，the expression of CD11b was significantly decreased in non heatsensitive moxibustion group and heat-sensitive moxibustion group(P＜0.05).Conclusion Heat-sensitive moxibustion can reduce the damage of cerebral inchemia-reperfusion，which might be through decreasing expression of CD11b.(www.xing528.com)

［Key words］moxa moxibustion； brain ischemia-reperfusion injury； CD11b； rats； tail-flick latency

脑血管病(cerebrovascular disease，CVD)具有较高的发病率、死亡率和致残率。在所有的脑血管病中，尤以缺血性脑血管病(ischemic cerebrovascular disease，ICVD)发病率最高，约占脑血管病总体发病率的60%～80%［1］，现临床上一般采用溶栓疗法［2］等血管再通技术使缺血性脑组织重新恢复血液灌注，有时反而可加重组织的功能障碍和结构损伤，即发生脑缺血再灌注损伤。研究表明，热敏灸对多种疾病有良好的疗效［3］，其治疗缺血性脑卒中也取得了较好的效果［4］。本课题组前期动物实验结果显示热敏灸能够缩小脑缺血再灌注损伤模型大鼠的脑梗死面积、减轻脑缺血再灌注损伤［5-8］。然而其作用机制尚不十分清楚。近年来，许多研究表明，炎症反应参与了脑缺血再灌注损伤的发病过程［8-9］。小胶质细胞(Microglia)是中枢神经系统的重要免疫细胞，CD11b是该细胞活化的重要标志物。研究报道，脑缺血再灌注数小时后，缺血侧CD11b阳性细胞表达明显增加［10-11］。那么，热敏灸减轻脑缺血再灌注损伤是否通过降低大鼠脑缺血再灌注损伤模型中CD11b表达、减轻炎症反应起作用还不十分清楚。本研究在前期工作的基础上，进一步探讨热敏灸减轻脑缺血再灌注损伤的作用机制，为热敏灸用于临床治疗缺血性卒中提供实验依据。