中药对更年期抑郁症大鼠模型5-HT系统的影响

［摘要］目的　制备更年期抑郁症大鼠模型，观察补益肝肾中药对模型大鼠海马5-羟色胺(5-HT)含量及其5-HT合成限速酶-色氨酸羟化酶2(TPH2)表达的影响，探讨补益肝肾中药防治更年期抑郁症的机理。方法　采用雌性SD大鼠，在卵巢切除术的基础上，联合孤养和21天慢性不可预见性温和刺激(CUMS)制备更年期抑郁症大鼠模型。以高效液相色谱-电化学检测法(HPLC-ECD)测定海马5-HT含量、RT-PCR技术检测中缝核TPH2 mRNA表达以及荧光免疫组化检测中缝核TPH2蛋白表达。结果　与模型组相比，中药组大鼠海马5-HT含量增加、中缝核TPH2 mRNA相对表达量增加以及TPH2阳性细胞数明显增多。结论　补益肝肾中药具有防治更年期抑郁症的作用，提高大鼠中缝核TPH2的表达，促进5-HT的合成，使海马5-HT含量升高，可能是补益肝肾中药防治更年期抑郁症的作用途径之一。

［关键词］更年期抑郁症；5-羟色胺；色氨酸羟化酶2

Effect of Chinese medicine of replenishing the liver and kidney on the system of 5-HT in perimenopausal depression model rats

［Abstract］Objective We established the perimenopausal depression rat model and observed the effect of Chinese medicine of replenishing the liver and kidney on the concentration of 5-hydroxytryptamine(5-HT)in hippocampus and the expression of tryptophan hydroxylase 2(TPH2)in median raphe nuclei in perimenopausal depression model rats in order to explore its mechanism.Methods The animal model was established by resecting the ovaries of female rats，then giving isolation-housing in combination with 21 days of chronic unexpected mild stress(CUMS).High performance liquid chromatogram-electrochemical detection(HPLC-ECD)were employed to measure 5-HT in hippocampus，the hemi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemistry were employed to detect the relative expression of TPH2 mRNA and number of TPH2 immunoreactive neurons in median raphe nuclei.Results After giving Chinese medicine 21 days，the concentration of 5-HT in hippocampus was increased in rats of the medicine group，the relative expression of TPH2 mRNA and number of TPH2 immunoreactive neurons in raphe nuclei were too.Conclusion Chinese medicine replenishing liver and kidney have effects of improving perimenopausal depression，increasing the expression of TPH2 in median raphe nuclei，which leads to enhance the synthesis of 5-HT in hippocampus might be one of its mechanisms.(www.xing528.com)

［Key words］perimenopausal depression；5-hydroxytryptamine；tryptophan hydroxylase 2

更年期抑郁症是诸多抑郁症中的一种类型，是特指初次发病于更年期(也称为围绝经期，是指从卵巢功能紊乱到绝经后1年)，以焦虑不安和情绪低落为主要症状的疾病，属于情感性精神障碍［1］。中药在临床上应用确能收到一定的疗效。本研究采用的补益肝肾中药是临床上用于治疗妇女更年期抑郁症的基础方，该方的基本组成为知母、黄柏、巴戟天、仙茅、仙灵脾等，临床上在辨证论治的基础上使用此方加减治疗妇女更年期抑郁症，具有较好的效果。然而该方的作用机理还不清楚。因此，本文采用雌性SD大鼠，在卵巢切除术的基础上，联合孤养和21天慢性不可预见性温和刺激(CUMS)制备更年期抑郁症大鼠模型，观察补益肝肾中药对更年期抑郁症大鼠模型的防治效果，并进一步观察它对海马5-羟色胺(5-HT)含量以及中缝核色氨酸羟化酶2(TPH2)表达的影响，以探讨其可能的作用机理。